MICROBIOTA OF THE RHIZOSPHERE OF THE SOWING ORDER FOR THE USE OF BIONEOSTIM AND VERMISTIM D

Abstract

The results of studies on the effect of the biological preparation Bioneostim (1.0 l/t) and the plant growth regulator Vermystym D (seed treatment before sowing – 7.0 l/t l/t, spraying of crops – 8.0 l/t l/ha) on the number of individual groups of microbiota of the rhizosphere of the seed bed (ammonifying, nitrifying, cellulolytic groups) are presented.The research was carried out in the field conditions of the Department of Biology of the Uman National University with the bookmark of researchers by a systematic method. Repetition – three times. The research scheme included options with pre- sowing treatment with Bioneostim at a rate of 1.0 l/t separately and in combination with Vermistim D (7.0 l/t – treatment and 8.0 l/ha – treatment of vegetative plants). The seeds of the seeding row one day before sowing were treated separately and with a mixture of Bioneostim and Vermistim D. Against the background of the treatment of the seeding row with Bioneostim and Vermistim D, the crops in the stemming phase were sprayed with the plant growth regulator Vermistim D at a rate of 8.0 l/ha.The number of microbiota in the rhizosphere of the seed coat was recorded in the flowering–bean formation phase. Soil samples were taken in accordance with generally accepted methods. Quantitative counting of ammonifying, nitrifying and cellulolytic microorganisms was performed on selective media prescribed in the relevant methods. The number of microorganisms was expressed in colony-forming units in 1 g of absolutely dry soil.The results of studies on the effect of the biopreparation Bioneostim, applied in different ways of applying the plant growth regulator Vermystym D, on the number of the main ecological and trophic groups of microorganisms in the rhizosphere of the seedbed, showed the dependence of their development on the combination of drugs, weather conditions and physiological characteristics of individual groups of bacteria. However, the greatest stimulating effect of drugs on the development of rhizosphere microbiota was observed when the biopreparation Bioneostim and the plant growth regulator Vermystym D were used together for seed treatment before sowing with the subsequent spraying of vegetative plants with Vermystym D. This composition provided an increase in the number of ammonifying bacteria in the rhizosphere of the seedbed by 13–61%, nitrifying bacteria by 26–62%, and cellulolytic bacteria by 40–53%. The increase in the number of individual groups of microorganisms in the rhizosphere of the seed bed with the combined use of biological preparations is consistent with the active course of physiological and biochemical processes in plants, due to which the influx of root secretions into the rhizosphere increases, which are a nutrient substrate for the microbiota.